Home > Miscellaneous Products > ELISA Kits and Antibodies > Infectious Disease Kits >

DRG Tetanus toxin (antitoxin) IgG ELISA, 96 wells
Tetanus toxin (antitoxin) IgG ELISA, 96 wells
Tetanus toxin (antitoxin) IgG ELISA, 96 wells


 
DRG Tetanus toxin (antitoxin) IgG ELISA, 96 wells
MUST SHIP NEXT DAY
Our Price: $163.80
Manufacturer: DRG International Inc.
Regular Price: $174.92
Shipping Weight: 5 lbs.

Product Code:
EIA3514

Description
 

Tetanus toxin IgG (EIA-3514)

Introduction

The DRG Tetanus toxin IgG Enzyme Immunoassay Kit for determination of IgG-class antibodies to Tetanus toxin in serum.

PRINCIPLE of the test

The DRG Tetanus toxin IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) Microtiter wells as a solid phase are coated with Tetanus toxoid antigen. Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation Tetanus toxin-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Tetanus toxin-specific IgG antibody in the specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.


Share your knowledge of this product with other customers... Be the first to write a review

Browse for more products in the same category as this item:

Miscellaneous Products > ELISA Kits and Antibodies > Infectious Disease Kits
Miscellaneous Products > ELISA Kits and Antibodies
Miscellaneous Products