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DRG RSV (Respir. Synth.Virus) IgA ELISA, 96 wells
RSV (Respir. Synth.Virus) IgA ELISA, 96 wells
RSV (Respir. Synth.Virus) IgA ELISA, 96 wells


 
DRG RSV (Respir. Synth.Virus) IgA ELISA, 96 wells
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Our Price: $147.00
Manufacturer: DRG International Inc.
Regular Price: $156.98
Shipping Weight: 5 lbs.

Product Code:
EIA3506

Description
 

Respiratory Syncytial Virus (RSV) IgA ELISA (EIA-3506)

Introduction

The DRG RSV IgA Enzyme Immunoassay Kit provides materials for determination of IgA-class antibodies to Respiratory Syncytial Virus (RSV) in serum. This assay is intended for research use only.

PRINCIPLE of the test

The DRG RSV IgA ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) Microtiter wells as a solid phase are coated with RSV antigen. Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation RSV-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgA antibodies are dispensed into the wells. During a second incubation this anti-IgA conjugate binds specifically to IgA antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of RSV-specific IgA antibody in the specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.


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