Guide: A Basic Overview Of Serum Protein Manual Agarose Electrophoresis
June 25th, 2019
Study: Detection Of Benzodiazepines In Oral Fluid By Homogeneous Enzyme Immunoassay
June 20th, 2019
MEDICA 2019 - World Forum for Medicine
Düsseldorf, GermanyBooth No.: 3/D35-2
AACC Annual Scientific Meeting and Clinical Lab Expo
Anaheim Convention Center ~ Anaheim, CABooth No.: 2627
Thanks to Block Scientific, I was able to procure the re-certified Bayer DCA 2000+ without hassles and get the lab back in operation. The
device works perfectly and I look forward to doing more business with Block Scientific.
--- Mathew Anderson, New Jersey
3rd Generation Enzyme Immunoassay (ELISA) for the determination of antibodies to Plasmodium species in human sera and plasma.
Recombinant proteins representing immunodominant epitopes of Plasmodium species, are coated onto wells of a microplate. Recombinant proteins have been carefully selected to ensure the screening of all antibodies to P. species. Serum or plasma samples are added to these wells and, if antibodies specific to P. species (IgG, IgM or IgA) are present in the sample, they will form stable complexes with the recombinant antigens in the well.
Antigen-antibody complexes are then identified through the successive addition of: (1) same biotinylated recombinant proteins specific to P. species and; (2) horseradish peroxidase HRP Streptavidin conjugate.
The hydrolytic activity of horseradish peroxidase allows for the quantification of these antibody-antigen complexes. Peroxidase substrate solution is then added. During incubation, a blue colour will develop in proportion to the amount of anti P. species antibodies bound to the well, thus establishing their presence or absence in the sample. Wells containing samples negative for anti-
P. species antibody remain colourless. A stop solution is added to each well and the resulting yellow colour is read on a microplate reader at 450 nm.