Home > Miscellaneous Products > ELISA Kits and Antibodies > Infectious Disease Kits >

DRG Malaria Ab ELISA, 96 Wells, Min 10 kits per order.
Malaria Ab ELISA, 96 Wells
Malaria Ab ELISA, 96 Wells, Min 10 kits per order


 
DRG Malaria Ab ELISA, 96 Wells, Min 10 kits per order
MUST SHIP NEXT DAY
Our Price: $285.60
Manufacturer: DRG International Inc.
Regular Price: $304.98
Shipping Weight: 15 lbs.

Product Code:
EIA5048

Description
 

DRG Malaria Ab Elisa (EIA-5048)

INTENDED USE

3rd Generation Enzyme Immunoassay (ELISA) for the determination of antibodies to Plasmodium species in human sera and plasma.

PRINCIPLE OF THE TEST

Recombinant proteins representing immunodominant epitopes of Plasmodium species, are coated onto wells of a microplate. Recombinant proteins have been carefully selected to ensure the screening of all antibodies to P. species. Serum or plasma samples are added to these wells and, if antibodies specific to P. species (IgG, IgM or IgA) are present in the sample, they will form stable complexes with the recombinant antigens in the well.

Antigen-antibody complexes are then identified through the successive addition of: (1) same biotinylated recombinant proteins specific to P. species and; (2) horseradish peroxidase HRP Streptavidin conjugate.

The hydrolytic activity of horseradish peroxidase allows for the quantification of these antibody-antigen complexes. Peroxidase substrate solution is then added. During incubation, a blue colour will develop in proportion to the amount of anti P. species antibodies bound to the well, thus establishing their presence or absence in the sample. Wells containing samples negative for anti- P. species antibody remain colourless. A stop solution is added to each well and the resulting yellow colour is read on a microplate reader at 450 nm.


Share your knowledge of this product with other customers... Be the first to write a review

Browse for more products in the same category as this item:

Miscellaneous Products > ELISA Kits and Antibodies > Infectious Disease Kits
Miscellaneous Products > ELISA Kits and Antibodies
Miscellaneous Products