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DRG Influenza B IgA ELISA, 96 wells
Influenza B IgA ELISA
Influenza B IgA ELISA, 96 wells


 
DRG Influenza B IgA ELISA, 96 wells
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Our Price: $135.80
Manufacturer: DRG International Inc.
Regular Price: $145.02
Shipping Weight: 5 lbs.

Product Code:
EIA4239

Description
 

Influenza Virus B IgA (EIA-4239)

Introduction

The DRG Influenza-Virus B IgA Enzyme Immunoassay Kit provides materials for the qualitative and semiquantitative determination of IgA-class antibodies to Influenza-Virus B in serum. This assay is intended for in vitro use only. In the United States, this kit is intended for Research Use Only.

PRINCIPLE of the test

The DRG Influenza-Virus B IgA ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) Microtiter wells as a solid phase are coated with Influenza-Virus B antigen. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Influenza-Virus B-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman IgA antibodies are dispensed into the wells. During a second incubation this anti-IgA conjugate binds specifically to IgA antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

The intensity of this color is directly proportional to the amount of Influenza-Virus B-specific IgA antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.


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