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DRG HBc Ab Screening, 96 wells
HBc Ab Screening, 96 wells
HBc Ab Screening, 96 wells


 
DRG HBc Ab Screening, 96 wells
MUST SHIP NEXT DAY
Our Price: $236.60
Manufacturer: DRG International Inc.
Regular Price: $252.66
Shipping Weight: 5 lbs.

Product Code:
EIA4086

Description
 

HBc Ab Screening, 96 wells

INTENDED USE

Competitive Enzyme ImmunoAssay (ELISA) for the determination of antibodies to Hepatitis B core Antigen in human plasma and sera. For research use only.

Principle Of The Test

The assay is based on the principle of competition where the antibodies in the sample compete with a monoclonal antibody for a fixed amount of antigen on the solid phase. A purified recombinant HBcAg is coated to the microwells.The donor’s serum/plasma is added to the microwell together with an additive able to block interferences present in the sample.

In the second incubation after washing, a monoclonal antibody, conjugated with Horseradish Peroxidase (HRP) and specific for HBcAg is added and binds to the free rec-HBcAg coated on the plastic.

After incubation, microwells are washed to remove any unbound conjugate and then the chromogen/substrate is added. In the presence of peroxidase enzyme the colorless substrate is hydrolyzed to a colored end-product. The color intensity is inversely proportional to the amount of antibodies to HBcAg present in the sample.


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