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DRG Fasciola ELISA, 96 wells
Fasciola ELISA, 96 wells
Fasciola ELISA, 96 wells


 
DRG Fasciola ELISA, 96 wells
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Our Price: $343.00
Manufacturer: DRG International Inc.
Regular Price: $366.28
Shipping Weight: 5 lbs.

Product Code:
EIA4503

Description
 

Fasciola ELISA, 96 wells

Introduction

The DRG Fasciola IgG Enzyme Immunoassay Kit provides materials for the qualitative and semiquantitative determination of IgG-class antibodies to Fasciola in serum. This assay is intended for in vitro use only.

Fasciola, also known as the common liver fluke or sheep liver fluke, is a parasitic flatworm of the class Trematoda, phylum Platyhelminthes that infects liver of a various mammals, including human. The disease caused by the fluke is called fascioliasis (also known as fasciolosis). Fasciola is world-wide distributed and causes great economic losses in sheep and cattle.

Principle Of The Test

The DRG Fasciola IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with Fasciola antigen from adult liver fluke. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Fasciolaspecific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

The intensity of this color is directly proportional to the amount of Fasciola-specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.


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