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DRG E. Coli Verotoxin 1+2 Ag (stool) ELISA, 96 wells
E. Coli Verotoxin 1+2 Ag (stool) ELISA, 96 wells
E. Coli Verotoxin 1+2 Ag (stool) ELISA, 96 wells


 
DRG E. Coli Verotoxin 1+2 Ag (stool) ELISA, 96 wells
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Our Price: $460.60
Manufacturer: DRG International Inc.
Regular Price: $491.86
Shipping Weight: 5 lbs.

Product Code:
EIA4452

Description
 

E. Coli Verotoxin 1+2 Ag (stool) ELISA, 96 wells

INTENDED USE

The DRG E. coli Verotoxin 1+2 Ag ELISA is an in vitro device for direct detection of verotoxin 1 and 2 (shiga-toxin 1 and 2) in faecal specimens and stool culture supernatants.

Principle Of The Test

The DRG E. coli Verotoxin 1+2 Ag ELISA is an indirect two-site-immunoassay for the qualitative determination of verotoxin 1 and 2 based on polyclonal and monoclonal antibodies. Verotoxin 1 and/or 2 of specimens and the positive control react with polyclonal anti-verotoxin 1 and 2 antibodies coated on the solid phase of the microplate. After incubation for 60 minutes at 22-25°C non-bound material is removed by a washing step.

Subsequently bound toxins specifically react with biotinylated monoclonal anti-verotoxin 1 and anti-verotoxin 2 antibodies during a second incubation period of 30 min at 22-25°C. Non-bound material is separated from the solid-phase immune complexes by a subsequent washing step.

During the next incubation period of 30 min at 22-25°C horseradish peroxidase (HRP) conjugated streptavidin reacts with the bound biotinylated antibodies. Unbound conjugate is removed by a washing step.


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