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DRG Syphilis (Treponema) Trep Screen (20 ul sample) ELISA, 96 wells
DRG Treponema pallidum Screen
Syphilis (Treponema) Trep Screen (20 μl sample) ELISA, 96 wells


 
DRG Syphilis (Treponema) Trep Screen (20 μl sample) ELISA, 96 wells
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Our Price: $183.40
Manufacturer: DRG International Inc.
Regular Price: $195.85
Shipping Weight: 5 lbs.

Product Code:
EIA5054

Description
 

Treponema pallidum Screen (EIA-5054)

INTRODUCTION

The DRG Treponema pallidum Screen Enzyme Immunoassay Kit provides materials for the qualitative determination of antibodies to Treponema pallidum in serum or plasma.

PRINCIPLE OF THE TEST

The DRG Treponema pallidum Screen ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with specific, recombinant treponemal antigens. Patient specimens and ready-for-use controls are pipetted into these wells. During incubation Treponema pallidumspecific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated treponemal antigens are dispensed into the wells. During a second incubation this conjugate binds specifically to antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Treponema pallidum-specific antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.


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