Guide: A Basic Overview Of Serum Protein Manual Agarose Electrophoresis
June 25th, 2019
Study: Detection Of Benzodiazepines In Oral Fluid By Homogeneous Enzyme Immunoassay
June 20th, 2019
MEDICA 2019 - World Forum for Medicine
Düsseldorf, GermanyBooth No.: 3/D35-2
AACC Annual Scientific Meeting and Clinical Lab Expo
Anaheim Convention Center ~ Anaheim, CABooth No.: 2627
Thanks to Block Scientific, I was able to procure the re-certified Bayer DCA 2000+ without hassles and get the lab back in operation. The
device works perfectly and I look forward to doing more business with Block Scientific.
--- Mathew Anderson, New Jersey
This ELISA is an in vitro immunoassay for the qualitative determination of Cryptosporidium antigen in fecal specimens.
Cryptosporidium is a coccidian parasite that is recognized as an important enteric pathogen. The organism causes an acute, though self-limiting infection in immunocompetent individuals. Incubation periods of 1 to 12 days have been reported with most oocyst shedding ending by day 21. Symptoms range from mild to severe diarrhea with a variety of complications. 1,8,9,10,11,13
The infection in immunocompromised patients is much more severe and may often be life threatening. Passage of fluid, up to 12 liters per day, has been reported. 1,2,3,12,14,16
Multiple pathways of Cryptosporidium transmission have been implicated. These include animal to human, water contamination and person-to-person. The latter may include contact between members of the same household, day care centers, and homosexual men. 1,2,12,14,16
Diagnosis of Cryptosporidium infections was done originally by direct detection techniques. Of these, microscopic examination of stools using stains or fluorescence labeled antibodies has been the most common. However, this method relies on an experienced technician and subsequent observation of intact organisms. Because of the historically low proficiency of correct microscopic examinations, alternative diagnostic methods have been investigated. 4,5,16,17
One important alternative has been the development of an antigen capture enzyme linked immunosorbent assay (ELISA) for use with stools. These tests, which have shown comparable sensitivity to experienced microscopic examinations, are fairly simple to perform and do not require the observation of intact organisms.