Home > Miscellaneous Products > ELISA Kits and Antibodies > Infectious Disease Kits >

DRG Chlamydia trachomatis IgG ELISA, 96 wells
Chlamydia trachomatis IgG ELISA, 96 wells
Chlamydia trachomatis IgG ELISA 96 wells


 
DRG Chlamydia trachomatis IgG ELISA 96 wells
MUST SHIP NEXT DAY
Our Price: $163.80
Manufacturer: DRG International Inc.
Regular Price: $174.92
Shipping Weight: 5 lbs.

Product Code:
EIA3462

Description
 

Chlamydia trachomatis IgG ELISA, 96 wells

Introduction

The DRG Chlamydia trachomatis IgG Enzyme Immunoassay Kit provides materials for the qualitative and semiquantitative determination of IgG-class antibodies to Chlamydia trachomatis in serum. This assay is intended for in vitro use only. In the United States, this kit is intended for Research Use Only.

Principle Of The Test

The DRG Chlamydia trachomatis IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) Microtiter wells as a solid phase are coated with recombinant Chlamydia trachomatis antigen. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Chlamydia trachomatis-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Chlamydia trachomatis-specific IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.


Share your knowledge of this product with other customers... Be the first to write a review

Browse for more products in the same category as this item:

Miscellaneous Products > ELISA Kits and Antibodies > Infectious Disease Kits
Miscellaneous Products > ELISA Kits and Antibodies
Miscellaneous Products