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DRG Chlamydia trachomatis IgA ELISA, 96 wells
Chlamydia trachomatis IgA ELISA, 96 wells
Chlamydia trachomatis IgA ELISA, 96 wells


 
DRG Chlamydia trachomatis IgA ELISA, 96 wells
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Our Price: $163.80
Manufacturer: DRG International Inc.
Regular Price: $174.92
Shipping Weight: 5 lbs.

Product Code:
EIA3461

Description
 

Chlamydia trachomatis IgA ELISA, 96 wells

Introduction

The DRG Chlamydia trachomatis IgA Enzyme Immunoassay Kit provides materials for the qualitative and semiquantitative determination of IgA-class antibodies to Chlamydia trachomatis in serum. This assay is intended for in vitro use only.

Principle Of The Test

The DRG Chlamydia trachomatis IgA ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Patient samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgGclass antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results.

Microtiter wells as a solid phase are coated with recombinant Chlamydia trachomatis antigen. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Chlamydia trachomatis-specific antibodies of positive specimens and controls are bound to the immobilized antigens.

After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgA antibodies are dispensed into the wells. During a second incubation this anti-IgA conjugate binds specifically to IgA antibodies resulting in the formation of enzyme-linked immune complexes.

After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.

The intensity of this color is directly proportional to the amount of Chlamydia trachomatis-specific IgA antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.


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