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Diazyme 25-OH Vitamin D Assay for Clinical Chemistry Analyzers
25-OH Vitamin D Assay for Clinical Chemistry Analyzers
Diazyme 25-OH Vitamin D Assay


 

Diazyme 25-OH Vitamin D Assay
Manufacturer: Diazyme
Shipping Info: Shipping Charges Extra

Product Code:
DZ688C-K

Description
 

25-OH Vitamin D Assay for Clinical Chemistry Analyzers

Product Features

Diazyme's 25-OH Vitamin D Assay for Clinical Chemistry Analyzers is a liquid-stable, three-reagent system. The assay requires only 20 uL of sample and returns patient results within 19 min. The assay has excellent correlation with leading 25-OH Vitamin D immunoassays (R2=0.984, slope=1.005, y-intercept= -0.210) and very good precision (within-run %CV are 2.2 to 14.2 and total %CV are 2.9 to 14.0).

Assay Principle

The test is based on the principle of α-complementation of the enzyme β-galactosidase and the competition between an enzyme donor-25-OH Vitamin D conjugate, an anti-Vitamin D antibody and the 25-OH Vitamin D content of a serum sample. Samples with higher 25-OH Vitamin D concentrations produce higher β-galactosidase activities and vice versa. A nitrophenyl-ß- galactoside derivative (NPG) is used as the enzyme substrate. The reaction's product has maximum absorbance at 415 nm. The 25-OH Vitamin D concentration of a sample is proportional to the measured β-galactosidase activity.

Intended use

The Diazyme 25-OH Vitamin D Assay is intended for use in clinical laboratories for the quantitative determination of 25-hydroxyvitamin D (25-OH-D) in human serum and plasma on automated chemistry analyzer. Measurement of 25-hydroxyvitamin D (25-OH-D) is for assessment of vitamin D sufficiency. For in vitro diagnostic use only.

Product:
Kit (80 Tests)

Catalog Number :
DZ688C-K

Packaging:
Diluent: 1 x 17 mL
R1: 1 x 8.5 mL
R2: 1 x 17 mL
R3: 1 x 8.5 mL
Cal: 5 x 1 mL
(Calibrator Included)

Method/Format:
Enzyme Immunoassay,
Liquid Stable


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