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  Toxoplasma gondii IgM ELISA*, 96 wells
Toxoplasma gondii IgM ELISA*, 96 wells
Toxoplasma gondii IgM ELISA*, 96 wells


 
Toxoplasma gondii IgM ELISA*, 96 wells
MUST SHIP NEXT DAY
Our Price: $210.00
Manufacturer: DRG International Inc.
Regular Price: $224.25
Shipping Weight: 5 lbs.

Product Code:
EIA3520

Description
 

DRG Toxoplasma gondii IgM (EIA-3520)


Introduction
The DRG Toxoplasma gondii IgM Enzyme Immunoassay Kit is for determination of IgM-class antibodies to Toxoplasma gondii in serum.
PRINCIPLE of the test
The DRG Toxoplasma gondii IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA).
Samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune antihuman
IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This
pretreatment avoids false negative or false positive results.
Microtiter wells as a solid phase are coated with detergent treated Toxoplasma gondii antigen (Deelen strain).
Pretreated specimens, ready-for-use standards and ready-for-use control are pipetted into these wells. During
incubation Toxoplasma gondii-specific antibodies of positive specimens, and standards are bound to the immobilized
antigens.
After a washing step to remove unbound sample, standard and control material horseradish peroxidase conjugated antihuman
IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to
IgM antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are
detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping
the enzymatic indicator reaction with sulfuric acid.
The intensity of this color is directly proportional to the amount of Toxoplasma gondii-specific IgM antibody in the
specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.


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