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The DRG PAPP-A ELISA is an enzyme immunoassay for measurement of Pregnancy associated plasma protein A (PAPP-A) in serum and plasma. This kit is intended for Research Use Only.
The DRG PAPP-A ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a polyclonal anti PAPP-A antibody. An aliquot of donor sample containing endogenous PAPP-A is incubated in the coated well with assay buffer.
After incubation the unbound material is washed off. In the second incubation step a sandwich complex is formed with a polyclonal anti PAPP-A antibody peroxidase conjugate. Having added the substrate solution, the intensity of color developed is proportional to the concentration of PAPP-A in the donor sample.
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