DRG Meningitis IgG (EIA-4918)

INTENDED USE
Enzyme ImmunoAssay (ELISA) for the determination of IgG antibodies to groups ACWY Meningococcus in human
plasma and sera.

PRINCIPLE OF THE TEST
Microplates are coated with a preparation of purified capsular polysaccharides formed by serogroups A, B, C, Y and
W135. In the 1st incubation, the solid phase is treated with diluted samples and anti-Meningococcus IgG are captured, if
present, by the antigens. After washing out all the other components of the sample, in the 2nd incubation bound anti-Men
IgG are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP). The enzyme captured on the solid
phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-
Men IgG antibodies present in the sample. A cut-off value permits to transform the optical density values detected in
positive or negative results due to the presence of absence of anti-Men IgG.