Malaria ELISA, 96 wells

INTENDED USE
These kits are intended for use by appropriately trained and qualified personnel for the detection of antibodies to
P.falciparum, P.vivax, P.ovale and P.malariae in human serum and plasma.

PRINCIPLE OF THE TEST
The Malaria ELISA use four recombinant antigens in a sandwich test to produce a test that is both highly specific and
sensitive. The antigens will detect P.falciparum, P.vivax, P.ovale and P.malariae -specific IgG, IgM, and IgA; enabling
the test to detect antibodies during all stages of infection. All reagents except the Conjugate and Wash solution are
supplied ready to use and colour-coded, and the procedure uses undiluted samples and standard volumes for ease of both
manual and automated use. The assay can be used with both serum and plasma.
The plastic wells are coated with a mixture of P. falciparum and P. vivax recombinant antigens. The antigenic similarity
between Plasmodium species means that antibodies to all species can be detected. Specific antibodies in serum or plasma
specimens combine with these antigens and with the same antigens conjugated to horseradish peroxidase, when conjugate
is added to a well in which the specimen has been incubated. After unreacted material has been removed by washing, the
presence of bound enzyme indicating the presence in the specimen of specific antibodies is revealed by a colour change in
the substrate/chromogen mixture. The intensity of the colour is compared to that in control wells to determine the
presence or absence of specific antibody.