DRG Malaria Ab Elisa (EIA-5048)

INTENDED USE
3rd Generation Enzyme Immunoassay (ELISA) for the determination of antibodies to Plasmodium species in human
sera and plasma.

PRINCIPLE OF THE TEST
Recombinant proteins representing immunodominant epitopes of Plasmodium species, are coated onto wells of a
microplate.
Recombinant proteins have been carefully selected to ensure the screening of all antibodies to P. species. Serum or
plasma samples are added to these wells and, if antibodies specific to P. species (IgG, IgM or IgA) are present in
the sample, they will form stable complexes with the recombinant antigens in the well.
Antigen-antibody complexes are then identified through the successive addition of: (1) same biotinylated
recombinant proteins specific to P. species and; (2) horseradish peroxidase HRP Streptavidin conjugate.
The hydrolytic activity of horseradish peroxidase allows for the quantification of these antibody-antigen complexes.
Peroxidase substrate solution is then added.
During incubation, a blue colour will develop in proportion to the amount of anti P. species antibodies bound to the
well, thus establishing their presence or absence in the sample. Wells containing samples negative for anti-
P. species antibody remain colourless.
A stop solution is added to each well and the resulting yellow colour is read on a microplate reader at 450 nm.