Human IL-33, 10 x 96 Wells

FOR RESEARCH USE ONLY.

Product Name: Human IL-33

Sample Type: Serum or plasma

# of Samples: 2X96 Assays

Introduction: Interleukin-1 (IL-1) family members, such as IL-19/: and IL-18, play important roles in host defense, immune regulation, neuronal injury and inflammation (1, 2). Recently, a new member of this family, IL-33, also named Nuclear Factor, from High Endothelial Venules (NF-HEV) (3), has been reported. It mediates its biological effects via IL-1 receptor ST2 (4), an orphan receptor of the IL-1 receptor family (5), and activates NF-kB and MAPK pathways. IL-33 is synthesized in response to cytokines such as IL-1: and plays an important role in TH2-associated immunology. IL-33 expression drives production of TH2-associated cytokines from in vitro polarized TH2 cells and induces the expression of IL-4, IL-5, and IL-13 in vivo. Overexpression of IL-33 in mice leads to severe pathological changes in mucosal organs. Structurally, IL-33 is closest to IL-18, rather than IL-1:. The human IL-33 is expressed with a prodomain (aa 1-111) that is cleaved by caspase-1 to produce a 18kDa protein (112-270) (6)

Storage and Stability: -20C

Product Name: Human IL-33

Range of Detection: 500 - 0.7 ng/ml

Shelf life: One year from date of receipt.

Assay Condition:The kit performance has been optimized for the stated protocol using the materialslisted and standard dilutions from 500-0.7 ng/ml of human IL-33. For alternativeassay conditions, the operator must determine appropriate dilutions of reagents.ELISA assay reactivity is sensitive to any variation in operator, pipetting andwashing techniques, incubation time and temperature, composition of reagents, andkit age. Adjustments may be required to position the standard curve and/or samplesin the desired detection range.

Country of Origin: United States of America

Assay Use: For Research Use Only (RUO). Not for diagnostic or therapeutic use in humans or animals. Not for animal or human consumption.