Herpes Simplex-2 IgG ELISA, 96 wellsIntroduction
The DRG Herpes simplex Virus Type 2 IgG Enzyme Immunoassay Kit provides materials for the qualitative and semiquantitative determination of IgG-class antibodies to Herpes simplex Virus Type 2 (HSV-2) in serum. This assay is intended for in vitro use only. In the United States, this kit is intended for Research Use Only.Principle Of The Test
The DRG HSV 2 IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA).
Microtiter wells as a solid phase are coated with HSV 2 antigen.
Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation
HSV 2-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
After a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman
IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds
specificly to IgG antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive
results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns
into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
The intensity of this color is directly proportional to the amount of HSV 2-specific IgG antibody in the patient
specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.