Block Scientific, Inc

Herpes Simplex 1+2 IgG ELISA, 96 wells

Introduction
The DRG Herpes Simplex Virus Type 1+2 IgG Enzyme Immunoassay Kit provides materials for the qualitative and semiquantitative determination of IgG-class antibodies to Herpes Simplex Virus Type 1+ 2 in human serum. This assay is intended for in vitro use only. In the United States, this kit is intended for Research Use.
Only.

Principle Of The Test
The DRG Herpes Simplex Virus Type 1+2 IgG ELISA Kit is a solid phase enzyme-linked immunosorbent
assay (ELISA)
Microtiter wells as a solid phase are coated with Herpes Simplex Virus Type 1 antigen.
Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation
Herpes Simplex Virus Type 1+2-specific antibodies of positive specimens and controls are bound to the
immobilized antigens.
After a washing step to remove unbound sample and control material horseradish peroxidase conjugated antihuman
IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds
specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive
results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns
into yellow by stopping the enzymatic indicator reaction with sulfuric acid.
The intensity of this color is directly proportional to the amount of Herpes Simplex Virus Type 1+2-specific
IgG antibody in the patient specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.