HEV Ab ELISA, 96 Wells, Min 10 kits per order

INTENDED USE
Third generation Enzyme Immunoassay (ELISA) for determination of antibodies to Hepatitis E Virus in human plasma
and sera. For Research Use Only.

Principle Of The Test
Microplates are coated with HEV-specific synthetic antigens encoding for conservative and immunodominant
determinants derived from Mexican and Burma virus strains.
The solid phase is first treated with the diluted sample and HEV Ab are captured, if present, by the antigens.
After washing out all the other components of the sample, in the 2nd incubation bound HEV antibodies, IgG and IgM as
well, are detected by the addition of polyclonal specific anti hIgG&M antibodies, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is
proportional to the amount of anti HEV antibodies present in the sample.
A cut-off value let optical densities be interpreted into HEV antibody negative and positive results.