HBc IgM ELISA Screening, 96 wells

INTENDED USE
An Enzyme ImmunoAssay (ELISA) for determination of IgM class antibodies to Hepatitis B Virus core Antigen in plasma
and sera with the "capture" system. For Research Use Only.

Principle Of The Test
The assay is based on the principle of "IgM capture" where IgM class antibodies in the sample are first captured by the
solid phase coated with anti hIgM antibody. After washing out all the other components of the sample and in particular IgG
antibodies, the specific IgM captured on the solid phase are detected by the addition of a purified preparation of
recombinant HBcAg, labelled with a monoclonal antibody conjugated with peroxidase (HRP). After incubation, microwells
are washed to remove unbound conjugate and then the chromogen/substrate is added. In the presence of peroxidase the
colorless substrate is hydrolyzed to a colored end product, whose optical density may be detected and is proportional to the
amount of IgM antibodies to HBcAg present in the sample.