HBc Ab Screening, 96 wells

INTENDED USE
Competitive Enzyme ImmunoAssay (ELISA) for the determination of antibodies to Hepatitis B core Antigen in human
plasma and sera. For research use only.

Principle Of The Test
The assay is based on the principle of competition where the antibodies in the sample compete with a monoclonal antibody
for a fixed amount of antigen on the solid phase.
A purified recombinant HBcAg is coated to the microwells.
The donor’s serum/plasma is added to the microwell together with an additive able to block interferences present in the
sample.
In the second incubation after washing, a monoclonal antibody, conjugated with Horseradish Peroxidase (HRP) and specific
for HBcAg is added and binds to the free rec-HBcAg coated on the plastic.
After incubation, microwells are washed to remove any unbound conjugate and then the chromogen/substrate is added. In
the presence of peroxidase enzyme the colorless substrate is hydrolyzed to a colored end-product.
The color intensity is inversely proportional to the amount of antibodies to HBcAg present in the sample.