Follicle-Stimulating Hormone (FSH), 1 x 96 Assays

FOR RESEARCH USE ONLY.

Product Name: Follicle-Stimulating Hormone (FSH)

Sample Type: Serum

No: of Samples: 1X96 Assays

Intended Use: For the quantitative determination of FSH concentration in human serum. The assay is useful in the diagnosis and treatment of pituitary and gonadal disorders

Principles of the assay: The GenWay Biotech FSH EIA Test is based on the principle of a solid phase enzyme-linked immunosorbent assay.13,14 The assay system utilizes mouse monoclonal anti-FSH for solid phase (microtiter wells) immobilization, and sheep anti-alpha FSH in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the antibodies, resulting in the FSH molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 45 minute incubation at room temperature, the wells are washed with water to remove unbound labeled antibodies. A solution of Tetramethylbenzidine (TMB) is added and incubated for 20 minutes at room temperature, resulting in the development of a blue color. The color development is stopped with the addition of 1N HCl, and the resulting yellow color is measured spectrophotometrically at 450 nm. The concentration of Follicle-Stimulating Hormone is directly proportional to the color intensity of the test sample.

Summary and Explanation of Test:  Storage and Stability: 1. Store the unopened kit at 2-8C upon receipt and when it is not in use, until the expiration shown on the kit label. Refer to the package label for the expiration date.
2. Keep microtiter plate in a sealed bag with desiccant to minimize exposure to damp air.

Storage and Stability: 1. Store the unopened kit at 2-8C upon receipt and when it is not in use, until the expiration shown on the kit label. Refer to the package label for the expiration date.
2. Keep microtiter plate in a sealed bag with desiccant to minimize exposure to damp air.

Limitation of the Test: 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and with adherence to good laboratory practice.
2. The results obtained from the use of this kit should be used only as an adjunct to other diagnostic procedures and information available to the physician.
3. The serum samples used in this assay must not contain any additives; EDTA has been found to interfere with the test procedure.
4. Serum samples demonstrating gross lipemia, gross hemolysis, or turbidiy should not b used with this test.
5. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.