Fasciola ELISA, 96 wells

Introduction
The DRG Fasciola IgG Enzyme Immunoassay Kit provides materials for the qualitative and semiquantitative
determination of IgG-class antibodies to Fasciola in serum.
This assay is intended for in vitro use only.
Fasciola, also known as the common liver fluke or sheep liver fluke, is a parasitic flatworm of the class Trematoda,
phylum Platyhelminthes that infects liver of a various mammals, including human. The disease caused by the fluke is
called fascioliasis (also known as fasciolosis). Fasciola is world-wide distributed and causes great economic losses in
sheep and cattle.

Principle Of The Test
The DRG Fasciola IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA).
Microtiter wells as a solid phase are coated with Fasciola antigen from adult liver fluke.
Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Fasciolaspecific
antibodies of positive specimens and controls are bound to the immobilized antigens.
After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human
IgG antibodies are dispensed into the wells. During a second incubation this anti-IgG conjugate binds specifically to
IgG antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results)
are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by
stopping the enzymatic indicator reaction with sulfuric acid.
The intensity of this color is directly proportional to the amount of Fasciola-specific IgG antibody in the patient
specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.