E. Coli Verotoxin 1+2 Ag (stool) ELISA, 96 wells

INTENDED USE
The DRG E. coli Verotoxin 1+2 Ag ELISA is an in vitro device for direct detection of verotoxin 1 and 2 (shiga-toxin 1
and 2) in faecal specimens and stool culture supernatants.

Principle Of The Test
The DRG E. coli Verotoxin 1+2 Ag ELISA is an indirect two-site-immunoassay for the qualitative determination of
verotoxin 1 and 2 based on polyclonal and monoclonal antibodies.
Verotoxin 1 and/or 2 of specimens and the positive control react with polyclonal anti-verotoxin 1 and 2 antibodies coated
on the solid phase of the microplate. After incubation for 60 minutes at 22-25°C non-bound material is removed by a
washing step.
Subsequently bound toxins specifically react with biotinylated monoclonal anti-verotoxin 1 and anti-verotoxin 2
antibodies during a second incubation period of 30 min at 22-25°C. Non-bound material is separated from the solid-phase
immune complexes by a subsequent washing step.
During the next incubation period of 30 min at 22-25°C horseradish peroxidase (HRP) conjugated streptavidin reacts with
the bound biotinylated antibodies. Unbound conjugate is removed by a washing step.