Dengue Igm ELISA, 96 wells

Introduction
The DRG Dengue Virus IgM Enzyme Immunoassay Kit provides materials for determination of IgM-class antibodies to Dengue Virus in serum. This kit is intended for Research Use Only.

Principle Of The Test
The DRG Dengue Virus IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA)
Donor samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyperimmune
anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid
factors. This pretreatment avoids false negative or false positive results.
Microtiter wells as a solid phase are coated with Dengue - 2 Virus antigen.
Diluted donor specimens and ready-for-use controls are pipetted into these wells. During incubation Dengue Virusspecific
antibodies of positive specimens and controls are bound to the immobilized antigens.
After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM
antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM
antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are
detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by
stopping the enzymatic indicator reaction with sulfuric acid.
The intensity of this color is directly proportional to the amount of Dengue Virus-specific IgM antibody in the donor
specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.