Boekel Scientific Analog Dry Bath Incubators for Diverse ApplicationsJune 27th, 2016
Point of Care Testing with Alere Cholestech LDX® SystemJune 24th, 2016
Medica 2016 – World Forum for Medicine
AACC Annual Meeting and Clinical Lab Expo - July 31 - August 4, 2016
Philadelphia, PA USA
Thanks to Block Scientific, I was able to procure the re-certified Bayer DCA 2000+ without hassles and get the lab back in operation. The
device works perfectly and I look forward to doing more business with Block Scientific.
--- Mathew Anderson, New Jersey
The DRG Cytomegalie Virus (CMV) IgM Enzyme Immunoassay Kit provides materials for determination of IgMclass antibodies to Cytomegalie Virus in serum. In the United States, this kit is intended for Research Use Only.
The DRG Cytomegalie Virus (CMV) IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) Donor samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyperimmune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results.
Microtiter wells as a solid phase are coated with inactivated Cytomegalie Virus antigen (strain 169). Pretreated donor specimens and ready-for-use controls are pipetted into these wells. During incubation Cytomegalie Virus-specific antibodies of positive specimens and controls are bound to the immobilized antigens.
After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specificly to IgM antibodies resulting in the formation of enzyme-linked immune complexes.
After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.The intensity of this color is directly proportional to the amount of Cytomegalie Virus-specific IgM antibody in the donor specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader.